Campylobacter jejuni is a Gram-negative microaerophilic bacterium that is the leading cause of food-borne gastroenteritis in developed countries. The common route of human infection is via consumption of poorly prepared or under-cooked poultry, in which C. jejuni is an asymptomatic commensal. Human disease is characterised by mild to severe inflammatory diarrhoea, vomiting and inflammation. C. jejuni has also been associated with post-infection immune-mediated complications such as Guillain-Barre Syndrome, reactive arthritis and irritable bowel syndrome. The molecular basis for C. jejuni infection includes initial adherence to, followed by invasion of, human intestinal epithelium; however there remains limited knowledge on both the molecular basis of pathogenesis and the host cell response. Human intestinal Caco-2 cells were co-cultured with the pathogenic chicken colonizing isolate C. jejuni NCTC11168 O during a time-course of infection. Multiplicity of infection (the ratio of bacteria to mammalian cells) was determined to elucidate the characteristics of C. jejuni adhesion to, and invasion of, intestinal cells. The infection efficiency (total vs invading), intracellular survival (viability after prolonged co-incubation) and the cytotoxicity of C. jejuni in Caco-2 cells were determined. A temporal profile of Caco-2 cell response to C. jejuni infection was examined by quantitative proteomics, glycoproteomics and glycome profiling using liquid chromatography and tandem mass spectrometry. The results and methodologies that were used to characterise C. jejuni-mediated exploitation of host cell biology will be presented and discussed.